DNA Sequencing
Zymo Research Corporation Fungal DNA Standards and No Template Control, Fungal DNA
Fungal DNA Standards and No Template Control (#1-8) for use with Femto Fungal DNA Quantification Kit.
New England Biolabs, Inc. CpG Methyltransferase (M.SssI) – 500 units
The CpG Methyltransferase, M.SssI, methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5'...CG...3'
Zymo Research Corporation RRHP 5-hmC Library Prep Kit 12 preps.
The RRHP 5-hmC Library Prep Kit is an all-inclusive solution for analysis of genome-wide 5-hydroxymethylcytosine (5-hmC) positions at single-base resolution. The Reduced Representation Hydroxymethylation Profiling (RRHP) method is based on blocking MspI digestion by glucosylating 5-hmC within MspI recognition sites. Fragments lacking glucosylated 5-hmC at the adapter-ligation junction will be cleaved and not amplified by PCR. Therefore, only fragments containing 5-hmC will be successfully amplified and analyzed by Next-Generation Sequencing. Fragments with higher 5-hmC levels will be correlated with higher frequency of sequencing reads. RRHP bypasses the need for bisulfite conversion, which allows for DNA inputs as low as 100 ng, lower sequencing depth, and straight-forward bioinformatics processing.
Seracare Life Sciences Inc Seraseq ctDNA Complete™ Mutation Mix AF1% | 1 x 25 µL
To further accelerate the validation and clinical application of liquid biopsy-based ccfDNA-targeted NGS assays, SeraCare has developed the Seraseq ctDNA Complete that expands coverage of all relevant variant types including SNVs, INDELs, CNVs, and gene fusions in a single highly-multiplexed reference sample. These new products are offered in either a purified ctDNA format or a plasma-like matrix format, all precisely quantitated by digital PCR against a single well-characterized genomic background (GM24385), and orthogonally validated with NGS technology. This specific ctDNA Complete product has an allele frequency of 1%.
ILEX LIFE SCIENCES LLC PAN-Biotech Mesenpan: xeno-free, serum-free medium for human mesenchymal stem cells (hMSC) expansion (500 ml kit)
Mesenpan: xeno-free, serum-free medium for human mesenchymal stem cells (hMSC) expansion (500 ml) QUANTITY: 1 kit. MANUFACTURED BY: Pan-Biotech (Germany). STORAGE: Media: 2 to 8 degrees Celsius; Supplement: -20 degrees Celsius. DESCRIPTION: Mesenpan Special Medium is a xeno-free, serum-free medium that was developed especially for proliferation of human mesenchymal stem cells (hMSC) without cell differentiation. It allows a long time culture from hMSC while preserving the differentiation potential in different cell types (multi-lineage potential). To reach a differentiation of the expanded hMSC into different lines, the suitable differentiation factors and protocols should be used. COMPOSITION: Media contains salts, amino acids, trace elements, hormones and growth factors in an optimized formulation. Kit includes 500 ml Mesenpan Basal Medium and 5 ml Mesenpan Growth Supplement.
Enzo Life Sciences AMPINEXT™ Post-Bisulfite DNA Library Preparation Kit (Illumina) (12Reactions)
The AMPINEXT™ Post-Bisulfite DNA Library Preparation Kit is a complete set of optimized reagents to prepare a DNA library -- after successful bisulfite conversion -- for various Illumina platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representation bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications. The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be quickly constructed using sub-nanogram input concentrations of DNA since the DNA is first bisulfite-converted and then used for library preparation. Assay Time: 5 hours.